Effect of Twist gene on the migration and invasion of gastric carcinoma cells.
- Author:
Geng-qiu LUO
1
;
Ji-fang WEN
;
Jing-he LI
;
Yong-bin HU
;
Chang-li ZHENG
;
Hai-ying JIANG
Author Information
1. Department of Pathology, School of Basic Medicine, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Cadherins;
biosynthesis;
Cell Movement;
genetics;
Humans;
Neoplasm Invasiveness;
Neoplasm Metastasis;
Stomach Neoplasms;
genetics;
metabolism;
pathology;
Transfection;
Tumor Cells, Cultured;
Twist-Related Protein 1;
biosynthesis;
genetics;
Vimentin;
biosynthesis
- From:
Journal of Central South University(Medical Sciences)
2008;33(2):174-178
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of Twist gene on the migration and invasion of human gastric carcinoma cells.
METHODS:MKN28 cells, a human gastric carcinoma cell line, were transfected with PcDNA3.1-Twist plasmid by lipofectamine transfecting technique. The transfected cells were selected with geneticin. Expressions of Twist,ecadherin and vimentin protein were detected by Western blot in cells transfected Twist gene. Matrigel invision chambers were performed to analyse the cell migration and invasion.
RESULTS:MKN28 cells transfected with PcDNA3.1-Twist plasmid showed stronger intracellular expression of Twist protein than MKN28 cells transfected with PcDNA3.1 and MKN28 cells without transfection. The expression of ecadherin protein in MKN28 cells transfected with PcDNA3.1-Twist plasmid was significantly decreased compared with that in MKN28 cells transfected with PcDNA3.1 and MKN28 cells without the transfection. However, The expression of vimentin protein in MKN28 cells transfected with PcDNA3.1-Twist plasmid was significantly increased compared with that in MKN28 cells transfected with PcDNA3.1 and MKN28 cells without transfection. The migration and invasion ability of Twist+ - MKN28 cells were stronger than that of MKN28 cells transfected with PcDNA3.1 and MKN28 cells without transfection.
CONCLUSION:Twist gene may promote the migration and invasion ability of gastric carcinoma cells through epithelial mesenchymal transition.
