Effect of IGF-1 on NO and PGE2 in rabbit articular chondrocytes induced by IL-1.
- Author:
Cheng PENG
1
;
Tao XIAO
;
Yuan-ming LUO
;
Xia-jun LIU
;
Mian-hui LIN
;
Jin-xi HU
Author Information
1. Research Institute of Traumatic Orthopaedics, Second Xiangya Hospital, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cartilage, Articular;
cytology;
metabolism;
Cells, Cultured;
Chondrocytes;
drug effects;
metabolism;
Dinoprostone;
metabolism;
Insulin-Like Growth Factor I;
pharmacology;
Interleukin-1;
pharmacology;
Nitric Oxide;
metabolism;
Osteoarthritis;
metabolism;
Rabbits
- From:
Journal of Central South University(Medical Sciences)
2008;33(3):197-203
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of insulin-like growth factor (IGF-1) on the concentration of NO and PGE(2) in the supernatant of rabbit articular chondrocytes induced by IL-1, and to explore the mechanism of IGF-1 in the development of osteoarthritis (OA).
METHODS:The samples were divided into 7 groups: IL-1beta 10 microg/L group, IL-1beta 10 microg/L+IGF-1 1 microg/L group, IL-1beta 10 microg/L+IGF-1 10 microg/L group, IL-1beta 10 microg/L+IGF-1 50 microg/L group, IL-1beta 10 microg/L+IGF-1 100 microg/L group, IGF-1 50 microg/L group, and a blank control group. The chondrocytes from the articular cartilage of 2 month old rabbits were cultivated and identified, and then co-cultured in the second filial generation chondrocytes on plates with or without recombinant human IGF-1 or IL-1. The concentration of NO was detected by nitrate reductase kit, and that of PGE(2) by enzyme-linked immunosorbent assay (ELISA). The results were analyzed by statistical method.
RESULTS:The average value of NO and PGE(2) was (89.971+/-10.224) micromol/L and (22.028+/-8.731) micromol/L in the IL-1beta 10 microg/L group, and (12.404+/-8.809) micromol/L and (1.900+/-0.227) ng/L in the blank control group. The concentration of NO and PGE(2) in IL-1beta 10 microg/L group was significantly higher than that in the blank control group (P<0.05). At the same concentration of 10 microg/L, IGF-1 could dose-dependently decrease the increase of NO and PGE(2) concentration induced by IL-1beta in the chondrocytes supernatant in vitro, and the optimum concentration of IGF-1 was 50 microg/L.
CONCLUSION:IL-1 can significantly increase the concentration of NO and PGE(2), and IGF-1 can dose-dependently decrease the concentration of NO and PGE(2) in the chondrocytes supernatant in vitro. The optimum concentration of IGF-1 was 50 microg/L.
