Expression of caveolin-1 and the invasion of choriocarcinoma.
- Author:
Hui-ning LIU
1
;
Jing-ting CAI
;
Qiu-hua LIN
;
Ke-ren HE
;
Rong YU
Author Information
1. Department of Obstetrics and Gynecology, Xiangya Hospital, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Caveolin 1;
biosynthesis;
genetics;
Choriocarcinoma;
metabolism;
pathology;
Female;
Humans;
Neoplasm Invasiveness;
RNA Interference;
RNA, Messenger;
biosynthesis;
genetics;
RNA, Small Interfering;
genetics;
Tumor Cells, Cultured;
Uterine Neoplasms;
metabolism;
pathology
- From:
Journal of Central South University(Medical Sciences)
2008;33(4):331-337
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the association between the expression of caveolin-1(CAV-1) and the invasion of choriocarcinoma, and to explore the effect of CAV-1 small interfering RNA(siRNA) on the invasion of choriocarcinoma cell line JEG-3.
METHODS:(1) Matrigel invasion assay and 3-(4,4)-dimethylthiahiazo (-z-yl)-3,5-di-phenytetrazoliumormide (MTT) assay were used to examine the difference in invasion and proliferation ability between JEG-3 cells and JAR cells;(2) Expression of caveolin-1 gene in the human chorionic villi tissues and chorionicnoma cell lines (JEG-3 cells and JAR cells) were detected by semi-quantitative RT-PCR. (3) The effect of CAV-1 siRNA transfection on the expression of CAV-1 mRNA, and the invasion and proliferation ability of JEG-3 cells were measured by RT-PCR, Matrigel invasion assay and MTT assay.
RESULTS:(1) The invasion ability of JEG-3 cell line was stronger than that of JAR cell line (P<0.05), but the difference in proliferation ability between JAR and JEG-3 was not obvious (P>0.05);(2) The expression of caveolin-1 gene in chorionicnoma cell lines was significantly stronger than that in the human normal chorion(P<0.05), and the expression of caveolin-1 gene in JEG-3 cells was stronger than that in the JAR cells (P<0.05). The data suggested that there was significantly positive correlation between caveolin-1 and the invasiveness of chorionicnoma cells (r=0.086,P<0.05);(3) CAV-1 siRNA could knock-out the expression of CAV-1 mRNA, and inhibit the invasion and proliferation ability of chorionicnoma cells.
CONCLUSION:CAV-1 can promote the invasion ability of chorionicnoma cells. CAV-1 siRNA can inhibit the invasion and proliferation ability of chorionicnoma cells.
