Biocompatibility of combined deproteinized bone coated with hepatocyte growth factor as scaffold for osteoblasts in vitro in fetal rabbits.
- Author:
Hong-sheng DANG
1
;
Meng ZHAO
;
Yong-xiang YAN
;
Jia-guo LIU
Author Information
1. Department of Orthopaedics, Taihe Hospital, Medical School of Yunyang, Shiyan Hubei, China. danghsh@tom.com
- Publication Type:Journal Article
- MeSH:
Animals;
Biocompatible Materials;
pharmacology;
Bone Substitutes;
metabolism;
Bone and Bones;
cytology;
Cell Proliferation;
Cells, Cultured;
Female;
Fetus;
Hepatocyte Growth Factor;
pharmacology;
Osteoblasts;
cytology;
Osteogenesis;
Pregnancy;
Rabbits;
Tissue Engineering;
methods;
Tissue Scaffolds
- From:
Journal of Central South University(Medical Sciences)
2008;33(4):359-364
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the cellular compatibility of combined deproteinized bone(DPB) coated with hepatocyte growth factor (HGF), and to observe the adherent effect of osteoblasts in response to HGF.
METHODS:Osteoblasts were isolated from fetal rabbits. Osteoblasts were cultured with DPB coated with HGF and deproteinized bone as experimental group and contral group, respectively. The proliferation and alkalinephosphatase activity were tested. Their growth was examined by inverted phase contrast microscope and scanning electronmicroscope.
RESULTS:The osteoblasts were attached to the outside and inside surfaces and grew well. HGF/DPB could stimulate the alkalinephosphatase activity of the osteoblasts and improve the proliferation of the osteoblasts.
CONCLUSION:HGF/DPB has good biocompatibility and bone induction. HGF could improve the adherent effect of DPB on osteoblasts, and it could be used as scaffold material for the bone tissue engineering.
