Screening for differentially expressed proteins in nasopharyngeal carcinoma by laser capture microdissection and proteomic analysis.
- Author:
Hong YI
1
;
Ai-lan CHENG
;
Wei-guo HUANG
;
Peng-fei ZHANG
;
Mao-yu LI
;
Fang PENG
;
Feng LI
;
Cui LI
;
Zhu-chu CHEN
;
Zhi-qiang XIAO
Author Information
1. Medical Research Center, Xiangya Hospital, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Antigens, Neoplasm;
isolation & purification;
Biomarkers, Tumor;
isolation & purification;
Carcinoma, Squamous Cell;
chemistry;
Electrophoresis, Gel, Two-Dimensional;
Humans;
Lasers;
Microdissection;
methods;
Molecular Sequence Data;
Nasopharyngeal Neoplasms;
chemistry;
Neoplasm Proteins;
isolation & purification;
Proteomics;
methods;
Serpins;
isolation & purification
- From:
Journal of Central South University(Medical Sciences)
2008;33(5):375-383
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To search for the differentially expressed proteins of nasopharyngeal carcinoma (NPC),and provide scientific evidence for identifying molecular biomarkers for NPC.
METHODS:Laser capture microdissection (LCM) was used to purify the target cells from NPC and normal nasopharyngeal epithelial tissues (NNET). Two-dimensional gel electrophoresis (2-DE) was used to separate the total proteins of microdissected NPC and NNET, PDQuest software was applied to analyze 2-DE images,and the differential proteins between the 2 types of tissues were identified by both MALDI-TOF-MS and ESI-Q-TOF-MS. Western blot and immunohistochemistry of tissue microarray were used to detect the expression of the differential protein SCCA1 in NPC and NNET.
RESULTS:2-DE patterns of microdissected NPC and NNEC were established,and 36 differential proteins in the NPC and NNEC were identified,20 of which only expressed or up-regulated in NPC and 16 only expressed or up-regulated in NNET. The differentially expressed level of SCCA1 in the NPC and NNET was confirmed by Western blot and immunohistochemistry of tissue microarray.
CONCLUSION:Thirty-six differentially expressed proteins identified in this study may be associated with the carcinogenesis of NPC,and may be candidate molecular biomarkers for NPC.
