Proteomic analysis of Helicobacter pylori in human gastritis and gastric cancer.
- Author:
Yun-hui YOU
1
;
Xue-gong FANG
;
Ping LIU
;
Hong-bo LIU
;
Xue-fei TIAN
;
Xue-mei YAN
Author Information
1. Department of Rheumatology and Immunology, Xiangya Hospital, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Bacterial Proteins;
analysis;
Electrophoresis, Gel, Two-Dimensional;
Female;
Gastritis;
microbiology;
Helicobacter Infections;
microbiology;
Helicobacter pylori;
Humans;
Male;
Proteome;
analysis;
Proteomics;
methods;
Stomach Neoplasms;
microbiology
- From:
Journal of Central South University(Medical Sciences)
2008;33(5):384-390
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish 2-dimensional electrophoresis (2-DE) maps of Helicobac-ter pylori in human gastritis, and gastric cancer, to identify the differentially expressed proteins,and to discuss the role of bacterial factor in pathogenesis.
METHODS:The total proteins of Helicobacter pylori in human gastritis and gastric cancer were separated by immobilized pH gradient-based 2-DE. The differentially expressed proteins were screened by PDQuest analysis software and identified by peptide mass fingerprint based on matrix-assisted laser desorption/ionization time of flight mass spectrometry, and searched on database.
RESULTS:A well-resolved and reproducible 2-DE pattern of Helicobacter pylori was obtained from patients with human gastritis and gastric cancer. Fourteen differentially expressed proteins were identified, including proteins related to anti-oxidation,molecular chape-rones and detoxification, enzymes related to metabolism,proteins related to cytoarchitecture,and proteins related to signal conduction.
CONCLUSION:A well-resolved and reproducible 2-DE pattern of Helicobacter pylori in human gastritis and gastric cancer is established and differentially expressed proteins from these 2 diseases are identified. The differentiation of protein expression may play an important role in the pathogenesis of gastric cancer.
