Effect of pinggan qianyang on hypothalamic proteome in the hyperthyroid rats with hyperactivity of liver-yang.
- Author:
Yao-hui YIN
1
;
Zhen-jia YI
;
Guang-wei ZHONG
;
Jian-jun HU
Author Information
1. Institute of Combined Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Drugs, Chinese Herbal;
therapeutic use;
Female;
Hyperthyroidism;
drug therapy;
metabolism;
Hypothalamus;
metabolism;
Male;
Medicine, Chinese Traditional;
Nerve Tissue Proteins;
metabolism;
Peroxiredoxin VI;
metabolism;
Phytotherapy;
Proteome;
metabolism;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Repressor Proteins;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2008;33(5):391-398
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of pinggan-qianyang (PGQY), a Chinese medicine, on hypothalamic proteome in the hyperthyroid rats with hyperactivity of liver-yang, and to explore its mechanism.
METHODS:The rat model was established by intraperitoneal injection of levo-thyroxine (L-T4) and fuzi decotion. All the quantitative and qualitative changes of the protein expressions were compared among the normal group,the model group and the treatment group by proteomic techniques.
RESULTS:The protein spots in the 3 groups were mainly displayed at the isoelectric point (pI) 3 approximately 10, and the molecular weights were 13.8 approximately 98.8 kD.Compared with the normal group, 6 spots of protein expression increased and 10 decreased in the model group. All the changed protein in the model group returned to normal level after PGQY treatment. Mass-spectrometer and bio-informatics indicated that these proteins were Prohibitin, Peroxiredoxin-6, histidine triad nucleotide-binding protein 1, protein-tyrosine-phosphatase, predicted protein, profilin-2, peroxir doxin-II, heat shock protein-27, and annexin-A1.
CONCLUSION:There are differences in the expression of hypothalamus proteins in the hyperthyroid rats with hyperactivity of liver-yang after the treatment with PGQY, and the 9 identified protein spots may be associated with the mechanism of PGQY.
