Polymorphism of vitamin D receptor Fok I and colorectal cancer risk in Chinese.
- Author:
Guo WANG
1
;
Bao-qun LI
;
Hong-hao ZHOU
Author Information
1. Institute of Clinical Pharmacology, Central South University, Changsha, China. wangguo30@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Colorectal Neoplasms;
genetics;
Female;
Genotype;
Humans;
Male;
Middle Aged;
Polymorphism, Genetic;
genetics;
Receptors, Calcitriol;
genetics;
Risk Factors
- From:
Journal of Central South University(Medical Sciences)
2008;33(5):399-403
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the frequency of vitamin D receptor (VDR) Fok I polymorphism in healthy Chinese and colorectal tumor patients,and to study the correlation between VDR Fok I polymorphism and the pathogenesis of colorectal tumor.
METHODS:After the preparation of gDNA by common method,VDR genotypes were determined by Fok I restriction endonuclease digestion of PCR-amplified DNA in 69 colorectal cancer patients and 218 healthy persons.
RESULTS:The F allele frequencies of VDR in healthy persons and in colorectal tumors patients were 61.5% and 49.3%, respectively, with statistical difference (P< 0.05). The genotype frequencies of FF, Ff and ff in healthy persons and in colorectal tumors patient were (39.5%,44%,16.5%) and (29.1%,40.5%,30.4%), respectively,with statistical differences (P< 0.05). The odds ratio of ff and Ff genotypes were 2.51 (95% confidence interval,1.21 approximately 5.18) and 2.00 (95% confidence intervals,1.01approximately 3.96), respectively (P< 0.01).
CONCLUSION:Fok I polymorphism is a common single nucleotide polymorphism (SNP) of VDR in Chinese population,and the VDR Fok I polymorphism may lower the risk of colorectal tumor.
