Histomorphologic properties of bovine jugular vein conduit treated withdye-mediated photooxidation following decellularization.
- Author:
Wei LI
1
;
Li-Juan YANG
;
Zhong-Shi WU
Author Information
1. Department of Cardiothoracic Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Bioprosthesis;
Blood Vessel Prosthesis;
Cattle;
Cell Separation;
Jugular Veins;
anatomy & histology;
transplantation;
ultrastructure;
Materials Testing;
Oxidants, Photochemical;
pharmacology;
Oxidation-Reduction;
Rats;
Rats, Wistar
- From:
Journal of Central South University(Medical Sciences)
2008;33(6):500-506
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the histocompatibility and histoconstancy of bovine jugular vein conduit (BJVC) treated by dye-mediated photooxidation following decellularization before and after implantation in Wistar rats.
METHODS:Each of 20 fresh bovine jugular veins with a retained native valve procured from a slaughterhouse was cut into 4 trial patches with valves, which were randomly divided into 4 groups. The 4 groups were treated respectively by dye-mediated photooxidation(DMP), glutaraldehyde(GA), decellularization(DC), and dye-mediated photooxidation following decellularization (DC+DMP). One of the trial patches in each group was implanted subcutaneously in the same Wistar rat. Two months later, all trial rats were killed and the specimens were retrieved. Tissue protein extraction was used to estimate the cross-linked degree of BJVC treated by dye-mediated photooxidation following decellularization. To observe the morphologic properties of the specimens, HE staining and electron microscopes were used.
RESULTS:Compared with others, the patches in the DC+DMP group were flexible, stretched, and relatively intact; lining endothelium was comparatively smooth; collagen fiber structure was slightly loose intact; and many cells were uniformly infiltrated in all layers.
CONCLUSION:BJVC treated by dye-mediated photooxidation following decellularization is superior to others in histocompatibility, and the rate of degradation can be regulated by the degree of dye-mediated photooxidation.
