Proteomic study of paclitaxel on human cervical carcinoma HCE1.
- Author:
Lan-Qin CAO
1
;
Qiu-Hua LIN
;
Xin LI
Author Information
1. Department of Gynecology and Obstetrics, Xiangya Hospital, Central South University, Changsha 410008, China. caolanqin@163.com
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents, Phytogenic;
pharmacology;
Biomarkers, Tumor;
analysis;
DNA-Binding Proteins;
analysis;
Eukaryotic Initiation Factor-1;
analysis;
Female;
Gene Expression Profiling;
Genome;
Humans;
Keratin-8;
analysis;
Paclitaxel;
pharmacology;
Phosphopyruvate Hydratase;
analysis;
Proteome;
analysis;
Proteomics;
methods;
Tumor Cells, Cultured;
Tumor Suppressor Proteins;
analysis;
Uterine Cervical Neoplasms;
metabolism;
pathology
- From:
Journal of Central South University(Medical Sciences)
2008;33(6):512-517
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the mechanism of paclitaxel on the protein expression of human cervical carcinoma cell line HCE1.
METHODS:The total proteins extracted from paclitaxel-treated HCE1 cells were analyzed by 2-dimensional gel electrophoresis (2-DE), and compared with those from untreated HCE1 cells. The differential proteins were identified by mass spectrometry. Western blot was used to determine the differential expression levels of the 2 proteins.
RESULTS:At 24 hour after paclitaxel (0.05 mumol/L) treatment, 2-DE images of paclitaxel-treated and paclitaxel-untreated cells were analyzed. Forty-two differential proteins were found. Twenty-one differential proteins among 42 proteins were analyzed by mass spectrometry, among which 15 proteins were identified, including peptidyl-prolylisomerases A (PPIase A),alpha-enolase,keratin 8,heat shock protein 90, eukaryotic translation initiation factor 1A, and so on.
CONCLUSION:Fifteen proteins in human cervical carcinoma cells paclitaxel-treated and paclitaxel-untreated are found by proteomic techniques. These proteins may be involved in the proliferation inhibition of human cervical carcinoma cells by paclitaxel.
