A preparative method of chronic experiment autoimmune encephalomyelitis.
- Author:
Jin-jin YAN
1
;
Guo-xiang HUANG
;
Bo XIAO
;
Huan YANG
;
Zhi-guo WU
;
Wen-bin ZHOU
Author Information
1. Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Disease Models, Animal;
Encephalomyelitis, Autoimmune, Experimental;
chemically induced;
Female;
Freund's Adjuvant;
Glycoproteins;
Mice;
Mice, Inbred C57BL;
Myelin-Oligodendrocyte Glycoprotein;
Peptide Fragments;
Random Allocation
- From:
Journal of Central South University(Medical Sciences)
2008;33(8):663-668
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the model of chronic experimental autoimmune encephalomyelitis (EAE)for the further study of multiple sclerosis.
METHODS:A total of 72 female SPF C57BL/6J mice (inbred strain, aged 8 approximately 10 weeks), were randomly divided into an EAE group, a blank group and an adjuvant group, and each group was divided into 3 subgroups: an onset group, a peak group and a chronic phase group. The EAE group was immunized with mMOG35-55.
RESULTS:At the end of the study, and 83.3% of the mice in EAE group suffered the onset, and 8.3% of the mice died. The highest clinical score reached grade 5, namely paralysis of the whole body and then death. In the EAE group, after being immunized first, the mice were all anosis during the first 13 days. They got ill on the third week, and in about 20 approximately 24 days the clinical symptom reached the peak, and in 28 approximately 32 days the chronic phase arrived,when parts of the clinical symptoms got relieved. On the contrary, both the adjuvant group and the blank group were healthy all the time. Characteristic appearance was detected in the EAE group.
CONCLUSION:Antigen emulsion, mixture of artificially synthesized mMOG35-55 and complete Freundos adjuvant can successfully induce chronic EAE in the mice. The model of EAE duplicated in our study has the characteristics of high incidence, low death rate and stability, which can be used to carry out further research on multiple sclerosis.
