Effect of sodium tungstate on glucose metabolism in adipocytes.
- Author:
Yan YANG
1
;
Zhao-hui MO
;
Ke CHEN
;
Hong-hui HE
;
Jing XIONG
Author Information
1. Department of Endocrinology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- MeSH:
3T3-L1 Cells;
Adipocytes;
metabolism;
Animals;
Glucose;
metabolism;
Glucose Transporter Type 4;
biosynthesis;
genetics;
Hypoglycemic Agents;
pharmacology;
Mice;
RNA, Messenger;
biosynthesis;
genetics;
Tungsten Compounds;
pharmacology;
Up-Regulation
- From:
Journal of Central South University(Medical Sciences)
2008;33(8):727-730
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of sodium tungstate on glucose metabolism in adipocytes and its mechanism.
METHODS:After 3T3-L1 preadipocytes were differentiated into adipocytes, these adipocytes were incubated with sodium tungstate (0, 150, 300, 500, and 700 micromol/L) for 48 h, and then glucose consumption of the adipocytes was detected by glucose-oxidase assay. Glucose transport was determined by the uptake of 2-deoxy-[3H]-D-glucose, and the expression of glucose transport-4 (GLUT-4) mRNA was identified by semi-quantitative RT-PCR.
RESULTS:Sodium tungstate (150 approximately 700 micromol/L) could significantly increase the glucose consumption and glucose transport with a concentration dependent-effect. Sodium tungstate could increase GLUT-4 mRNA expression.
CONCLUSION:Sodium tungstate can enhance the glucose metabolism of adipocytes by up-regulating the expression of GLUT-4 mRNA.
