Expression and significance of inflammatory factors and bone formation mediators in carotid atherosclerotic plaque.
- Author:
Wei ZHENG
1
;
Hong KANG
;
Chang SHU
;
Mei-lai TANG
;
Pei-zhi FANG
;
Jing XIE
;
Jie HE
;
Meng WANG
Author Information
1. Department of General Surgery II, Peopleos Hospital of Hunan, Changsha 410005, China. weizheng62003@yahoo.com
- Publication Type:Journal Article
- MeSH:
Atherosclerosis;
complications;
metabolism;
pathology;
Bone Morphogenetic Protein 6;
metabolism;
Calcinosis;
metabolism;
pathology;
Carotid Stenosis;
etiology;
metabolism;
pathology;
Chemokine CCL2;
metabolism;
Endarterectomy, Carotid;
Humans;
Inflammation;
metabolism;
pathology;
Interleukin-8;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2008;33(8):746-750
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To compare protein levels of pro-inflammatory factors and bone formation mediators in the fibrous cap and shoulder region of non-calcified and calcified carotid endarterectomy (CEA) plaques.
METHODS:Twenty-two CEA plaques were classified as non-calcified and calcified groups (n=11 each) in accordance with the American Heart Association (AHA) consensus in 1995. To make frozen sections and H&E staining using plaque, the mean percent of carotid stenosis and calcification area was determined by quantitative histomorphometry. The protein levels of pro-inflammatory interleukin-8 (IL-8), monocyte chematactic protein-1 (MCP-1), bone formation mediators bone morphogenetic protein-6 (BMP-6), and osteocalcin in the fibrous cap and shoulder region of plaques were determined by western blot and were quantified using ImageJ software.
RESULTS:MCP-1 and IL-8 protein were 1.3 (P>0.05) and 1.5 (P<0.05) folds greater in the non-calcified plaques than those in the calcified plaques. BMP-6 and osteocalcin protein were 1.3 (P>0.05) and 2.1 (P<0.01) folds greater in the calcified plaques compared with those of the non-calcified plaques.
CONCLUSION:Inflammation is more likely to occur in non-calcified carotid plaques, and calcification in the plaques may be associated with bone formation, which indicates that decreased inflammation may be the beginning of calcification in carotid atherosclerotic plaques.
