Expression of PDCD5 in multiple myeloma and its relation with BCL-2.
- Author:
Tie-Bin JIANG
1
;
Xin LI
;
Jun ZHOU
;
Yang ZHOU
;
Hong YUAN
;
Hui XIANG
;
Guo-Ping YANG
;
Hong-Wei LÜ
;
Xiao-Wei XING
;
Jing LIU
Author Information
1. Department of Hematology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Apoptosis;
genetics;
Apoptosis Regulatory Proteins;
biosynthesis;
genetics;
Bone Marrow Cells;
metabolism;
pathology;
Female;
Humans;
Male;
Middle Aged;
Multiple Myeloma;
genetics;
metabolism;
pathology;
Neoplasm Proteins;
biosynthesis;
genetics;
Proto-Oncogene Proteins c-bcl-2;
biosynthesis;
genetics;
RNA, Messenger;
biosynthesis;
genetics
- From:
Journal of Central South University(Medical Sciences)
2008;33(9):814-820
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the expression of apoptosis related gene PDCD5 in multiple myeloma (MM), and to analyze the relation between PDCD5 and BCL-2.
METHODS:The expressions of PDCD5 and BCL-2 protein and mRNA were determined by immunohistochemical staining method, flow cytometry (FCM) and reverse transcription polymerase chain reaction (RT-PCR) method in bone marrow mononuclear cells. We also analyzed the relation between PDCD5 and BCL-2.
RESULTS:Immunohistochemical staining showed that PDCD5 protein positive cell percentage, staining intensity index (SII) of PDCD5 protein, BCL-2 protein positive cell percentage, and SII of BCL-2 protein were (34.75 +/- 6.49)%, (281.16 +/- 75.33), (29.97 +/- 5.57)%, and (224.94 +/- 57.72) in the MM group and (52.98 +/- 5.84)%, (462.84 +/- 39.77), (5.56 +/- 1.95)%, and (27.84 +/- 9.75) in the control group (all P < 0.05). Results of FCM showed that PDCD5 protein positive percentage and mean fluorescence intensity of PDCD5 were (78.11 +/- 21.63)% and (61.73 +/- 11.04) in the MM group and (89.46 +/- 9.98)% and (353.04 +/- 123.26) in the control group (all P < 0.05). RT-PCR showed that relative expression of PDCD5 and BCL-2 mRNA were (0.33 +/ -0.07) and (0.33 +/- 0.08) in the MM group and (0.53 +/- 0.05) and (0.12 +/- 0.02) in the control group (all P < 0.05). The positive cell percentage of PDCD5 and BCL-2 protein was negative correlation (r = -0.86, P < 0.05); the expression of PDCD5 and BCL-2 mRNA was the same status (r = -0.90, P < 0.05).
CONCLUSION:The expressions of PDCD5 protein and mRNA in MM patients are down-regulated, but the expressions of BCL-2 protein and mRNA are up-regulated. The mRNA and protein expression of PDCD5 and BCL-2 has negative correlation.
