Hyperlipidemia induced by high fat diet ingestion activates TGF-beta/Smad signaling pathway in the kidney of diabetic rats.
- Author:
Zhou-xiong CHEN
1
;
Xiao-yun XIE
;
Ri-chen YU
;
Jun ZHANG
;
Min-xiang LEI
Author Information
1. Department of Endocrinology, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Diabetes Mellitus, Experimental;
complications;
metabolism;
Diabetic Nephropathies;
metabolism;
Dietary Fats;
administration & dosage;
Female;
Hyperlipidemias;
complications;
metabolism;
Kidney;
metabolism;
Rats;
Rats, Sprague-Dawley;
Signal Transduction;
Smad Proteins;
metabolism;
Transforming Growth Factor beta1;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2008;33(10):906-912
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of diet-induced hyperlipidemia on TGF-beta/Smad signaling pathway in the kidney of diabetic rats, and to explore the mechanism by which hyperlipidemia leads to renal injury in diabetes.
METHODS:Diabetic rats and non-diabetic rats were fed with normal fat diet and high fat diet for 16 weeks, respectively. The expressions of TGF-beta1, TbetaRII, and Col-IV mRNA in the renal cortex were examined by reverse transcriptase-PCR,TbetaRII and p-Smad staining in glomerular cells were detected by immunohistochemical staining, and the expression of TGF-beta1 and Col-IV protein was determined by Western blot.
RESULTS:Diet-induced hyperlipidemia up-regulated the levels of TGF-beta1, TbetaRII, p-Smad, and Col-IV protein and mRNA in the renal cortex of diabetic rats compared with those of non-diabetic rats. However, feeding high fat diet to non-diabetic rats had no influence on the expression of TGF-beta1, TbetaRII, p-Smad2, and Col-IV in the renal cortex.
CONCLUSION:Hyperlipidemia induced by high fat diet ingestion leads to renal injury in diabetic rats through activating TGF-beta1 /Smad signaling pathway.
