Effect of mycophenolate mofetil on the expression of early inflammatory reaction in diabetic rats.
- Author:
Lin PENG
1
;
Hao ZHANG
;
Guo XU
;
Ren-hong TANG
Author Information
1. Department of Nephrology, Third Xiangya Hospital,Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Chemokine CCL5;
biosynthesis;
genetics;
Diabetes Mellitus, Experimental;
drug therapy;
metabolism;
Diabetic Nephropathies;
metabolism;
prevention & control;
Ectodysplasins;
biosynthesis;
genetics;
Inflammation;
metabolism;
Kidney;
metabolism;
Male;
Mycophenolic Acid;
analogs & derivatives;
therapeutic use;
RNA, Messenger;
biosynthesis;
genetics;
Random Allocation;
Rats;
Rats, Sprague-Dawley
- From:
Journal of Central South University(Medical Sciences)
2008;33(10):913-918
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of mycophenolate mofetil(MMF) on early inflammatory reaction of renal lesion in streptozotocin(STZ)-induced diabetic rats.
METHODS:Thirty-six male Sprague-Dawley rats were randomly divided into 3 groups after uninephrectomy: normal control group, diabetic model group, and MMF-treated group. Six rats in each group were sacrificed at the 4th week and 14th week after STZ injection. Twenty-four hour urinary protein (24 h Upro) count was measured before death. The expressions of regulated on activation of normal T expressed and secreted (RANTES),ectodermal dysplasia (ED-1)and Col-IV protein in the renal tissue were detected by immunohistochemistry. The expression of RANTES mRNA in the renal tissue was detected by RT-PCR.
RESULTS:MMF prevented the increasing of 24h Upro in diabetic rats,and the expressions of RANTES,ED-1,Col-IV protein and RANTES mRNA in the kidney of MMF-treated rats were significantly decreased.
CONCLUSION:MMF plays an early renal protective role in diabetic nephropathy, possibly through inhibition of early inflammatory reaction.
