Differential proteomic analysis of nasopharyngeal carcinoma cell lines 5-8F and 6-10B.
- Author:
Ya-li XIANG
1
;
Hong YI
;
Cui LI
;
Peng-fei ZHANG
;
Mao-yu LI
;
Zhu-chu CHEN
;
Zhi-qiang XIAO
Author Information
1. Key Laboratory of Cancer Proteomics of Ministry of Health of China, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Carcinoma;
Cell Line, Tumor;
Electrophoresis, Gel, Two-Dimensional;
Humans;
Mass Spectrometry;
Nasopharyngeal Carcinoma;
Nasopharyngeal Neoplasms;
metabolism;
Proteome;
metabolism;
Proteomics
- From:
Journal of Central South University(Medical Sciences)
2007;32(6):978-984
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To compare the proteome difference of nasopharyngeal carcinoma (NPC) cell lines 5-8F and 6-10B, and to screen these proteins associated with NPC metastasis.
METHODS:Two-dimensional gel electrophoresis (2-DE) was used to separate the total proteins from NPC cell lines 5-8F and 6-10B with different metastatic potentials and same genetic background, respectively. PDQuest software was applied to analyze 2-DE images, and the differentially expressed protein spots between 5-8F and 6-10B were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The expression levels of partial identified proteins in the 2 cell lines were detected by Western blot.
RESULTS:2-DE maps of total proteins from 5-8F and 6-10B were established. A total of 65 differential protein spots in the 2 cell lines were found, and 15 non-redundant differential expression proteins were identified by MALDI-TOF-MS. Western blot showed that Annexin A1 and 14-3-3 protein sigma were differential expression proteins in 5-8F and 6-10B, which was consistent with the Results from the comparative proteomic analysis.
CONCLUSION:Fifteen non-redundant differential expression proteins are useful for studying the metastatic mechanism of NPC.
