Effects of NGX6 on the transcriptional activation of beta-catenin/TCF/LEF in Wnt/beta-catenin signal pathway.
- Author:
Fen LIU
1
;
Shou-rong SHEN
;
Hong-tao LI
;
Xiao-yan WANG
;
Ya PENG
;
Man-tian LIAO
;
Qin GUO
Author Information
1. Department of Gastroenterology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors;
genetics;
COS Cells;
Cell Line, Tumor;
Chlorocebus aethiops;
Genes, Reporter;
Humans;
Membrane Proteins;
genetics;
Transcription Factor 4;
Transcription Factors;
genetics;
Transcriptional Activation;
Transfection;
Tumor Suppressor Proteins;
genetics;
Wnt Signaling Pathway;
beta Catenin;
genetics;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2007;32(6):985-991
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effects of NGX6 on the transcriptional activation of beta-catenin/TCF/LEF in Wnt/beta-catenin signal pathway, and to identify the role of NGX6 in Wnt signal pathway.
METHODS:The eukaryotic expression vector pcDNA3.1(+)-beta-catenin (WT) was constructed. pcDNA3.1(+)-beta-catenin (WT) and pCMV-myc-NGX6 were cotransfected to COS-7 and the transcriptional activity of TCF/LEF was detected by TCF-4 luciferase report system. Without extro-genous beta-catenin, pCMV-myc-NGX6 was transfected alone to COS-7 and colon cancer cell line SW620, and the transcriptional activity of TCF/LEF was detected by TCF-4 luciferase report system, and then the expression of nucleus beta-catenin and TCF-4 was detected by Western blot.
RESULTS:The eukaryotic expression vector pcDNA3.1(+)-beta-catenin (WT) was successfully constructed. The activation of TCF-4 luciferase report gene in the cotransfection group in COS-7 was less than that in NGX6 alone transfection group (P<0.05). The activation of TCF-4 luciferase report gene in NGX6 alone transfection group without extro-genous beta-catenin was less than that in pCMV-myc transfection group in COS-7 and SW620. The expression of beta-catenin and TCF-4 was decreased after the NGX6 transfection in COS-7 and SW620 cells.
CONCLUSION:NGX6 can inhibit the transcriptional activation of beta-catenin/TCF/LEF in Wnt signal pathway by its negative regulation in the nuclear translocation of beta-catenin.
