Sequence polymorphism of the promoter region of gene STK11 in patients with Peutz-Jeghers syndrome.
- Author:
Xin YI
1
;
Mao-Jin YAO
;
Yong-Jun WANG
;
Jian-Guang TANG
;
Wen-Feng NING
;
Xiang-Ping WANG
;
Shi-Quan ZHOU
;
Chen-Jie LI
;
Fei WANG
;
Kun XIA
;
Xiao-Liu SHI
Author Information
1. Department of Gastroenterology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Base Sequence;
Gene Frequency;
Genotype;
Humans;
Molecular Sequence Data;
Peutz-Jeghers Syndrome;
genetics;
Polymorphism, Genetic;
Promoter Regions, Genetic;
genetics;
Protein-Serine-Threonine Kinases;
genetics
- From:
Journal of Central South University(Medical Sciences)
2007;32(1):74-77
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the relationship between the sequence variation of the promoter region (-1543 approximately -1160) of STK11 gene and the risk of developing Peutz-Jeghers syndrome (PJS).
METHODS:The sequences of the promoter region of 14 PJS patients (7 patients are inherited and the other 7 patients are sporadic) and 42 normal individuals were PCR amplified and then sequenced.
RESULTS:A new single nucleotide polymorphism (SNP) G/T (-1275) in STK11 promoter region was identified. The frequency of genotype GG, GT, and TT was 53.3%, 26.7%, and 20%, respectively among PJS patients and 33.3%, 64.3%, and 2.4%, respectively among the normal individuals. The frequency of genotype GG and TT among patients was significantly higher than that among the normal individuals, and the frequency of genotype GT among patients was significantly lower than that among the normal individuals (chi(2)=8.521, P<0.05).
CONCLUSION:G/T(-1275) in STK11 promoter region is a new SNP. The genotype of this new SNP may relate to the risk of developing Peutz-Jeghers syndrome (PJS) deserve further research.
