Experimental study of acute lung injury induced by glutamate in vivo.
- Author:
Li SHEN
1
;
Zi-Qiang LUO
;
Jian-Zhong HAN
;
Shao-Jie YUE
;
Xiao-Qun QIN
;
Chen LI
;
Hui-Jun LIU
Author Information
1. Department of Physiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
- Publication Type:Journal Article
- MeSH:
Acute Disease;
Animals;
Bronchoalveolar Lavage Fluid;
cytology;
Dizocilpine Maleate;
pharmacology;
Erythrocyte Count;
Glutamic Acid;
administration & dosage;
toxicity;
Leukocyte Count;
Lung Diseases;
chemically induced;
metabolism;
prevention & control;
Male;
Mice;
Receptors, N-Methyl-D-Aspartate;
antagonists & inhibitors;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2007;32(1):78-81
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the possible injury induced by glutamate in the lung.
METHODS:The lung wet weight/body weight (LW/BW), lung wet/dry weight (W/D), the content of cells and the total protein (TP) in bronchoalveolar lavage fluid (BALF) were determined together with the micromorphology observation.
RESULTS:(1) The LW/BW, W/D, the content of white blood cells, red blood cells and TP in BALF increased in a dose dependent manner 2 hours after the administration of the glutamate (0.50 - 0.75 g/kg). (2) Examination of histological sections showed the presence of lung inflammation charactered by neutrophils recruitment 2 hours after the glutamate administration. (3) The increase of W/D caused by glutamate (0.50 g/kg) was nearly abolished by pre-treatment with MK801 (a specific blocker of NMDA receptor, 0.1 mg/kg) for 30 minutes (P<0.05).
CONCLUSION:Glutamate can cause the acute lung injury through the activation of NMDA receptor in vivo.
