The Effects of Interleukin-17 on Production of Matrix Metalloproteinase-3 in Cultured Rheumatoid Arthritis Synoviocytes.
- Author:
Sun RYU
1
;
Sung Il KIM
Author Information
1. Medical Research Institute, Pusan National University Hospital, Busan, Korea. ksimd@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Rheumatoid arthritis synoviocytes;
MMP-3;
IL-17
- MeSH:
Arthritis, Rheumatoid*;
Enzyme-Linked Immunosorbent Assay;
Humans;
Interleukin-17*;
Joints;
RNA, Messenger
- From:The Journal of the Korean Rheumatism Association
2004;11(1):1-6
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To investigate the effect of interleukin-17 (IL-17) on the production of matrix metalloproteinase-3 (MMP-3) and the expression of MMP-3 mRNA in rheumatoid arthritis synoviocytes. METHODS: Fibroblast-like synovial cells (FLS) were prepared from the synovial tissues of rheumatoid arthritis patients and cultured in the presence of various concentration of IL-17. The production of MMP-3 and expression of MMP-3 mRNA were determined by sandwitch ELISA and real-time quantitative RT-PCR. RESULTS: In ELISA, stimulation of FLS by IL-17 with 5, 10, 20, 40 ng/mL concentrations increased the production of MMP-3 by 2.7, 2.9, 3.1, 3.6 fold after 24 hours and 9.9, 10, 11, 12 fold after 48 hours over the constitutive levels of unstimulated FLS. In real-time quantitative RT-PCR, stimulation of FLS by IL-17 with 5, 10, 20, 40 ng/mL concentrations increased the MMP-3/beta-actin mRNA ratio by 3.2, 5.7, 9.9, 30.5 fold after 24 hours over the constitutive levels of unstimulated FLS. CONCLUSIONS: IL-17 may be involved in the joint destruction in rheumatoid arthritis by enhancing the production of MMP-3 from rheumatoid arthritis synoviocytes.
