Inhibition of cell growth and induction of apoptosis in human hepatoma cell line HepG2 by tanshione IIA.
- Author:
Zhi-Hong ZHONG
1
;
Wen-Gui CHEN
;
Yong-He LIU
;
Qi-Xing LI
;
Yue QIU
Author Information
1. Medical School of Hunan Normal University, Changsha 410006, China.
- Publication Type:Journal Article
- MeSH:
Abietanes;
Antineoplastic Agents, Phytogenic;
pharmacology;
Apoptosis;
drug effects;
Carcinoma, Hepatocellular;
genetics;
pathology;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
DNA Fragmentation;
drug effects;
Dose-Response Relationship, Drug;
Drugs, Chinese Herbal;
pharmacology;
Flow Cytometry;
Humans;
Liver Neoplasms;
genetics;
pathology;
Microscopy, Fluorescence;
Phenanthrenes;
pharmacology;
Time Factors
- From:
Journal of Central South University(Medical Sciences)
2007;32(1):99-103
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the effect of tanshinone IIA on the growth and apoptosis in human hepatoma cell line HepG2.
METHODS:The human hepatoma cell line HepG2 was treated with tanshinone IIA at various concentrations for 72 h. The inhibition of proliferation was measured by MTT assay and apoptosis-related alterations in morphology measured by cytochemical staining (HT33258). DNA fragmentation was evaluated by agarose gel electrophoresis. Apoptotic rate and cell arrest were quantified by flow cytometry (FCM).
RESULTS:Tanshinone IIA inhibited the growth of HepG2 in a time- and dose- dependent manner. The semi-inhibitory concentration (IC50) value after the treatment with tanshinone IIA on HepG2 for 24, 48 and 72 h were 14.7, 7.4, and 3.9 microg/ mL, respectively. After the treatment with 0.5 - 10 microg/mL tanshinone IIA for 72 h, the formation of apoptotic bodies was observed. DNA ladder was shown in agarose gel electrophoresis, in addition to the cells treated by 1.0 microg/mL tanshinone IIA . The apoptotic rates at 0.5, 1.0, 2.0, 5.0, and 10.0 microg/mL for 72 h were 20.32%+/-2.16%, 28.0%+/-2.35%, 33.87%+/-3.43%, 46.73%+/-4.08% and 57.85%+/-3.74%, respectively, which were all significantly higher than those of the control group (P<0.05).
CONCLUSION:Tanshinone IIA can inhibit the proliferation of human hepatoma cell line HepG2 in a time- and dose- dependent manner, and the mechanism of growth inhibition of human hepatoma cells may be related to the induction of apoptosis.
