Antisense c-fos oligonucleotides-induced myopia in guinea pigs.
- Author:
Shuang-Zhen LIU
1
;
Xin WEI
;
Jie-Yue WANG
;
Xiao-Ying WU
;
Xing-Ping TAN
Author Information
1. Xiangya Hospital, Central South University, Changsha 410008, China. liushzhxy@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
Genes, Immediate-Early;
genetics;
Guinea Pigs;
Immunohistochemistry;
Microinjections;
Myopia;
chemically induced;
genetics;
physiopathology;
Oligonucleotides, Antisense;
administration & dosage;
genetics;
toxicity;
Proto-Oncogene Proteins c-fos;
biosynthesis;
genetics;
RNA, Messenger;
genetics;
metabolism;
Random Allocation;
Retina;
metabolism;
Reverse Transcriptase Polymerase Chain Reaction;
Signal Transduction;
physiology
- From:
Journal of Central South University(Medical Sciences)
2007;32(1):132-137
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To characterize the antisense c-fos oligonucleotides that control the expression of immediate-early gene c-fos in retina in order to better understand the mechanism by which antisense c-fos oligonucleotides induced myopia. In this study the signal transduction in the pathway linking visual experience and the regulation of the eye's growth was investigated.
METHODS:Thirty-one 3-week guinea pigs were assigned into 3 groups: antisense and sense c-fos oligonucleotides were intravitreally injected every 3 days to the eyes of the experimental guinea pigs at different concentrations; and saline vehicle to control guinea pigs in the same way. The refraction and axial length of the eyes were measured before and after the treatment, and the immediate-early gene c-fos expression in the retina was quantified by immunohistochemistry and RT-PCR.
RESULTS:The moderate myopia was induced in high (1 nmol) and low (0.1 nmol) level of antisense c-fos oligonucleotide intravitreous injection (-5.425 D and -5.575 D, respectively) compared with the control ateral eyes. The refraction and axial length of the treated eyes increased, and the expression of immediate-early gene c-fos decreased significantly in the antisense c-fos oligonucleotides intravitreously injected eyes compared with the sense c-fos oligonucleotide intravitreously and saline vehicle injected eyes (P<0.01). The refraction and axial length were of no statistically significant differences among the sense c-fos oligonucleotides-treated eyes and saline-treated eyes and non-treated eyes (P>0.05).
CONCLUSION:The obvious myopia can be induced by antisense c-fos oligonucleotides in guinea pigs; antisense c-fos oligonucleotides inhibit c-fos expression in the retina. Immediate-early gene c-fos may be a potential factor in the prevention of myopia and plays an important role in the signal transduction of the retina.
