Function and gene expression of the active efflux transporters in drug-resistant Candida albicans.
- Author:
Hui-Jun GUO
1
;
Zhong-di XIA
;
Gao-Li WU
;
Hui ZHOU
Author Information
1. Department of Microbiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
- Publication Type:Journal Article
- MeSH:
Antifungal Agents;
pharmacology;
Biological Transport;
Candida albicans;
genetics;
physiology;
Drug Resistance, Fungal;
genetics;
Fluconazole;
pharmacology;
Fungal Proteins;
genetics;
physiology;
Gene Expression Regulation, Fungal;
Membrane Transport Proteins;
genetics;
physiology;
Microbial Sensitivity Tests;
RNA, Messenger;
biosynthesis;
genetics;
Reverse Transcriptase Polymerase Chain Reaction;
Rhodamines;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2007;32(1):183-187
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the function and gene expression of active efflux transporters in drug-resistant candida albicans.
METHODS:The broth microdilution method was performed to determined the minimal inhibitory concentration of 4 antifungal drugs to 20 candida albicans. We compared the efflux of Rhodamine 6G between sensitive and some of fluconazole-resistant candida albicans, and screened out the resistant strains with significantly increased efflux of Rhodamine 6G. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to measure the mRNA levels of active efflux gene CDR1 and CDR2.
RESULTS:The efflux of Rhodamine 6G was significantly increased in some fluconazole-resistant strains when glucose was added, and the gene expressions of CDR1 and CDR2 were also obviously increased, compared with those in the sensitive strains.
CONCLUSION:The excessive expression of active efflux pump gene is related to the resistance to fluconazole in candida albicans. The measurement of Rhodamine 6G efflux is a useful method for the identification of drug-resistant strains induced by the excessive expression of active efflux pump.
