PTX1 in nasopharyngeal carcinoma by RNAi technology.
- Author:
Wen ZHOU
1
;
Hong LI
;
Xiang-Ling FENG
;
Lei WANG
;
Bin ZHU
;
Hui LI
;
Kai-Tai YAO
;
Cai-Ping REN
Author Information
1. Cancer Research Institute, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
genetics;
physiology;
Carcinoma, Squamous Cell;
genetics;
pathology;
Cell Cycle;
genetics;
physiology;
Cell Line, Tumor;
Flow Cytometry;
Gene Expression Regulation, Neoplastic;
Humans;
Nasopharyngeal Neoplasms;
genetics;
pathology;
RNA Interference;
RNA, Small Interfering;
genetics;
Reverse Transcriptase Polymerase Chain Reaction;
Transfection;
Vesicular Transport Proteins;
genetics;
physiology
- From:
Journal of Central South University(Medical Sciences)
2007;32(2):235-240
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the expression and the role of PTX1 located at the amplified 12p12-p11 region in nasopharyngeal carcinoma (NPC).
METHODS:Semi-quantitative RT-PCR and real-time RT-PCR were applied to detect the expression level of PTX1 in 36 NPC and 8 chronic nasopharyngitis (NP) biopsies. RNAi vector targeting PTX1 was constructed and transfected into NPC cell line 6-10B. The RNAi effect was determined by detecting the expression level of PTX1 in transfected 6-10B cell line. Finally, the cell biological characteristics were compared between transfected 6-10B and parental 6-10B by analyzing the cell cycle distribution and apoptosis status using flow cytometry.
RESULTS:RT-PCR and real-time RT-PCR revealed that PTX1 gene was over-expressed in NPC tissues (P<0.05). PTX1 expression was suppressed in NPC cell line 6-10B by approximately 65% by RNAi, confirmed by RT-PCR. The depletion of PTX1 could effectively block the proliferation and induce the apoptosis of NPC cells.
CONCLUSION:Blocking the expression of PTX1 on mRNA level changed the characterization of NPC cell line 6-10B by RNAi, suggesting that PTX1 identified in the amplified 12p12-p11 region may be involved in the genesis and development of NPC via promoting the cell proliferation and inhibiting the cell apoptosis.
