Azoospermia factor microdeletion on Y chromosome in patients with idiopathic azoospermia or severe oligozoospermia.
- Author:
Lan-Ping HU
1
;
Neng-Hui LIU
;
Qian PAN
;
De-Sheng LIANG
;
Zhi-Gao LONG
;
Hao HU
;
Hai-Yan ZHU
;
He-Ping DAI
;
Fang CAI
;
Ling-Qian WU
;
Kun XIA
;
Jia-Hui XIA
Author Information
1. National Laboratory of Medical Genetics of China, Xiangya Hospital, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Azoospermia;
genetics;
Chromosome Deletion;
Chromosomes, Human, Y;
genetics;
Genetic Loci;
Humans;
Infertility, Male;
diagnosis;
genetics;
Karyotyping;
Male;
Oligospermia;
genetics;
Seminal Plasma Proteins;
genetics
- From:
Journal of Central South University(Medical Sciences)
2007;32(2):241-245
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the correlation between male infertility and Y chromosome microdeletions of azoospermia factor (AZF) regions, and to establish a reliable genetic diagnosis in idiopathic infertile male patients with azoospermia or severe oligozoospermia.
METHODS:Multiplex PCR amplification of 6 sequence-tagged sites in AZF regions of the Y chromosome was examined among 100 normal karyotype male patients with azoospermia or oligozoospermia.
RESULTS:Four patients (4%) had Y chromosome microdeletions, the microdeletions of 3 patients were idiopathic azoospermic and those of the other 1 patient were secretory azoospermia.
CONCLUSION:The PCR-based Y chromosome microdeletion screening is simple and effective in the diagnosis of patients with severe male infertility. Microdeletion of Y chromosome is one of the major causes of severe dyszooospermia.
