Establishment of 2-dimensional electrophoresis maps of peripheral blood mononuclear cells.
- Author:
Bai-Yun ZHONG
1
;
Xue-Gong FAN
;
Wei-Dong LIU
;
Yi-Xuan YANG
;
Yun-Hui YOU
Author Information
1. Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Carcinoma, Hepatocellular;
blood;
Electrophoresis, Gel, Two-Dimensional;
methods;
Female;
Humans;
Leukocytes, Mononuclear;
cytology;
metabolism;
Liver Neoplasms;
blood;
Male;
Middle Aged;
Proteomics;
methods
- From:
Journal of Central South University(Medical Sciences)
2007;32(2):299-303
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the 2-dimensional electrophoresis(2-DE) profiles of peripheral blood mononuclear cells(PBMC) in patients with hepatocellular carcinoma(HCC) and health adults.
METHODS:The total proteins from PBMC in patients with HCC and healthy adult were separated by immobilized pH gradient-based 2-DE. The differential expression proteins were analyzed by PDQuest analysis software.
RESULTS:The well-resolved, reproducible 2-DE patterns of PBMC in patients with HCC and healthy adults were obtained. For HCC, the average spots of 2-DE maps were 1 206 +/- 48, and the average matching rate was 90.8%. For normal adults, the average spots were 1 123 +/- 37, and the average matching rate was 92.6%.
CONCLUSION:The well-resolved, reproducible 2-DE patterns of PBMC in patients with HCC and healthy adults are established. These proteomic analysis methods are useful to screen the potential biomarkers in the early diagnosis, treatment and prognosis monitor in patients with malignant tumor.
