Effects of endothelial cells from cord blood on the amplification of human early hematopoietic cells from cord blood in vitro.
- Author:
Li-Ming YIN
1
;
La-Mei CHENG
;
Qi-Ru WANG
;
Meng-Qun TAN
Author Information
1. Research Laboratory of Blood Physiology, Xiangya School of Medicine, Central South University, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Antigens, CD34;
analysis;
Cell Proliferation;
drug effects;
Coculture Techniques;
Culture Media;
pharmacology;
Cytokines;
pharmacology;
Endothelial Cells;
cytology;
metabolism;
Fetal Blood;
cytology;
metabolism;
Flow Cytometry;
Hematopoietic Stem Cells;
cytology;
metabolism;
Humans;
Immunohistochemistry
- From:
Journal of Central South University(Medical Sciences)
2007;32(2):304-308
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the effects of endothelial cells from umbilical cord blood (UCB) on the amplification of human early hematopoietic cells from UCB in vitro.
METHODS:Endothelial cells from UCB were cultured by the optimized medium of endothelial cells. There were 2 experiment groups: cytokines group (SCF+IL-3+IL-6+GM-CSF, CKs group) and noncontact group (endothelial cell layer with CKs without contacting the CD34+ cells group). CD34+ cells from UCB were isolated by MiniMACS. After the cells in the CKs group and the noncontact group were cultured for 7 days, the amplifying folds of early hematopoietic cells were assayed.
RESULTS:Early hematopoietic cells from UCB were expanded in the CKs group or the noncontact group. The amplifying folds of the noncontact group on early hematopoietic cells were significantly more than those of the CKs group.
CONCLUSION:The amplification effect of the noncontact group on early hematopoietic cells is superior to that of the CKs group.
