Effect of compound Rhizoma Smilacinus granules on the expression of Bcl-2 and Bax gene in A549 cell lines of non-small cell lung cancers.
- Author:
Guang-Cheng LI
1
;
Ke-Li DONG
;
Can-Hong HU
Author Information
1. Department of Traditional Chinese Medicine, Third Xiangya Hospital, Central South Universty, Changsha, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antineoplastic Agents, Phytogenic;
pharmacology;
Carcinoma, Non-Small-Cell Lung;
genetics;
pathology;
Drugs, Chinese Herbal;
pharmacology;
Gene Expression Regulation, Neoplastic;
drug effects;
Lung Neoplasms;
genetics;
pathology;
Male;
Proto-Oncogene Proteins c-bcl-2;
genetics;
RNA, Messenger;
biosynthesis;
genetics;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Reverse Transcriptase Polymerase Chain Reaction;
Serum;
bcl-2-Associated X Protein;
genetics
- From:
Journal of Central South University(Medical Sciences)
2007;32(2):312-315
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the effect of compound Rhizoma Smilacinus granules (CRSG) on the expression of Bcl-2 and Bax gene in A549 cell lines, and to explore the mechanism of CRSG on non-small cell lung cancers.
METHODS:The SD rats were randomly divided into 2 groups: one was administrated with CRSG (n=15), and the other with the same dose of distilled water (n=15). The herbage-containing serum was obtained 2 hours after the 6th treatment. non-small lung cancer A549 cell lines were randomly divided into CRSG group, diamminedichloroplatinum (DDP) group and normal control group, which were cultivated with 10% herbage-containing serum, DDP (3g/mL), and 10% SD rats serum respectively. The cultivated cells were collected after 48 hours, and then RT-PCR technique was used to determine the expression of Bcl-2 and Bax mRNA.
RESULTS:Compared with the normal control group after 48 h, the level of Bcl-2 mRNA and the rate of Bc-2/Bax decreased in the CRSG group and the DDP group, and the level of Bax mRNA increased with significant difference (P<0.01).
CONCLUSION:non-small cell lung cancer A549 cell lines have a high level of Bcl-2 mRNA and a low level of Bax mRNA. CRSG can significantly down-regulate the expression of Bcl-2 mRNA and the rate of Bc-2/Bax, and obviously up-regulate the expression of Bax mRNA, which probably is one of the molecular mechanisms of CRSG in inhibiting the growth of non-small cell lung cancers.
