Dicer efficiently converts large dsRNAs into siRNAs suitable for COX-2 gene.
- Author:
Hong LUO
1
;
Dong-xu HU
;
Ping CHEN
Author Information
1. Department of Respiratory Diseases Second Xiangya Hospital, Central South University, Changsha 410011, China. luohong1003@163.com
- Publication Type:Journal Article
- MeSH:
Base Sequence;
Cell Line, Tumor;
Cyclooxygenase 2;
genetics;
Humans;
Molecular Sequence Data;
RNA Interference;
RNA, Double-Stranded;
genetics;
metabolism;
RNA, Small Interfering;
genetics;
metabolism;
Ribonuclease III;
metabolism;
Sequence Analysis, DNA
- From:
Journal of Central South University(Medical Sciences)
2007;32(3):437-442
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To report the use of Dicer to cleave double-stranded RNA (dsRNAs) into small interference RNAs (D-siRNAs) that can target multiple sites within an mRNA, and to acquire an new method to cure inflammation of the airway and tumor.
METHODS:Using RiboMAX Large Scale RNA Production Systems-SP6 and T7 kit were used to transcribe A549 cell COX-2 DNA into RNA (dsRNAs). We mixed dsRNAs with Dicer in the reaction buffer. We recovered siRNAs using RNA Purification Column.
RESULTS:Dicer efficiently converted double-stranded RNA of COX-2 into small interference RNAs of 21 approximately 23 bp.
CONCLUSION:Dicer efficiently converts double-stranded RNA (dsRNA) into small interference RNAs (D-siRNAs of 21 approximately 23 bp).
