Inhibitive effect of troglitazone on TGF-beta(1) and fibronectin expression in human peritoneal mesothelial cells.
- Author:
Hong LIU
1
;
You-ming PENG
;
Fu-you LIU
;
Ying-hong LIU
;
Ling-yan LI
;
Jun LI
;
Xing CHEN
Author Information
1. Department of Nephrology, Second Xiangya Hospital, China.
- Publication Type:Journal Article
- MeSH:
Blotting, Western;
Cells, Cultured;
Chromans;
pharmacology;
Dose-Response Relationship, Drug;
Enzyme-Linked Immunosorbent Assay;
Epithelial Cells;
cytology;
drug effects;
metabolism;
Fibronectins;
biosynthesis;
genetics;
Glucose;
pharmacology;
Humans;
PPAR gamma;
biosynthesis;
genetics;
Peritoneum;
cytology;
RNA, Messenger;
biosynthesis;
genetics;
Reverse Transcriptase Polymerase Chain Reaction;
Thiazolidinediones;
pharmacology;
Transforming Growth Factor beta1;
biosynthesis;
genetics;
Troglitazone
- From:
Journal of Central South University(Medical Sciences)
2007;32(3):473-479
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of the peroxisome proliferator activated receptor-gamma (PPAR-gamma) agonist troglitazone on TGF-beta(1) and fibronectin (Fn) expression in human peritoneal mesothelial cells (HPMCs).
METHODS:HPMCs were cultured from human omentum by an enzyme digestion method, growing in medium containing 30 mmol/L D-glucose. TGF-beta(1) and Fn expression were measured in HPMCs in the presence and absence of 15 micromol/L troglitazone. The mRNA expressions of PPAR-gamma,TGF-beta(1) and Fn were determined by semi-quantification reverse transcriptive PCR (RT-PCR). The protein of TGF-beta(1) was determined by enzyme-linked immunosorbent assay (ELISA) and proteins of PPAR-gamma and Fn were determined by Western blot.
RESULTS:The mRNA and protein expression of TGF-beta(1) and Fn were significantly increased in HPMCs stimulated with 30 mmol/L D-glucose compared with the control group with F12 media (P<0.01). Obvious decrease of TGF-beta(1) was found in troglitazone(15 micromol/L) treated group compared with group stimulated with 30 mmol/L D-glucose (P<0.05). Exposure of HPMCs to troglitazone reduced the Fn secretion (P<0.05).
CONCLUSION:Troglitazone reduced the expression of TGF-beta(1) in HPMCs stimulated by 30mmol/L D-glucose, and reduced Fn production. PPAR-gamma agonists may have a specific role in ameliorating the course of progressive peritoneal fibrosis under long-term peritoneal dialysis states.
