Effects of Tongxinluo on cell viability and tissue factor in AngII induced vascular endothelial cells.
- Author:
Qi-lin MA
1
;
Ming SUN
;
Tian-lun YANG
;
Yuan-jian LI
;
Can-e TANG
;
Zhen-yu PENG
;
Shi-lin HE
;
Fang-ping CHEN
Author Information
1. Department of Cardiology, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Angiotensin II;
pharmacology;
Cell Line;
Cell Survival;
drug effects;
Cells, Cultured;
Drugs, Chinese Herbal;
pharmacology;
Endothelium, Vascular;
cytology;
drug effects;
metabolism;
Enzyme Inhibitors;
Enzyme-Linked Immunosorbent Assay;
Humans;
NG-Nitroarginine Methyl Ester;
pharmacology;
Nitric Oxide Synthase Type I;
antagonists & inhibitors;
biosynthesis;
genetics;
RNA, Messenger;
biosynthesis;
genetics;
Receptor, Angiotensin, Type 1;
biosynthesis;
genetics;
Reverse Transcriptase Polymerase Chain Reaction;
Thromboplastin;
biosynthesis;
genetics
- From:
Journal of Central South University(Medical Sciences)
2007;32(3):485-489
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the effects of Tongxinluo on cell viability and tissue factor (TF) in AngII induced vascular endothelial cells and to investigate its mechanism.
METHODS:AngII(10(-6)mol/L) was added to human vascular endothelial cells (HUVECs) culture media alone or with various concentration of Tongxinluo drug containing plasma (5%,10%, and 20%) added 30 minutes before AngII. Cell viability was evaluated after 24-hour incubation with AngII in a dose manner. TF, AngII type 1 receptor (AT(1)) mRNA, NO synthase (NOS) and NO were observed after 24-hour incubation with AngII. In addition, NOS inhibitor nomega-nitro-larginine (L-NAME) was added 30 minutes before Tongxinluo and AngII. Cell viability, TF, AT(1)mRNA, the level of NOS and NO were evaluated after 24-hour incubation with Tongxinluo and AngII.
RESULTS:Tongxinluo significantly improved AngII induced endothelial cell viability and the effect was the most obvious at 10%. Tongxinluo (10%) decreased the TF and AT(1) mRNA while increased the NOS and NO levels. L-NAME obviously inhibited the effects of Tongxinluo on cell viability, TF, AT(1) mRNA, and NOS and NO levels.
CONCLUSION:Up-regulating NOS-NO signaling may be the mechanism of Tongxinluo on cell viability and TF in AngII induced vacular endothelial cells.
