Culture of chondrocytes using nano-hydroxyapatite/collagen in vitro.
- Author:
Tao XIAO
1
;
Cheng PENG
;
Yuan-Ming LUO
;
Mian-Hui LIN
;
Xia-Jun LIU
Author Information
1. Research Institute of Traumatic Orthopaedics, Second Xiangya Hospital, Central South University, Changsha 410011, China. xiaotaoxyl@163.com
- Publication Type:Journal Article
- MeSH:
Cells, Cultured;
Chondrocytes;
cytology;
Collagen;
Durapatite;
Humans;
Prostheses and Implants;
Tissue Engineering;
methods;
Tissue Scaffolds
- From:
Journal of Central South University(Medical Sciences)
2007;32(4):641-645
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To evaluate the value of the nano-hydroxyapatite/ collagen (nHAC) as a scaffold for cartilage tissue engineering.
METHODS:The normal cartilage from patients after total hip arthroplasty of osteoarthritis was selected, and then chondrocytes were digested, separated, and amplificate in vitro. The chondrocytes were seeded onto the nHAC scaffold and were cultured in a 3-dimensional environment for 5 and 10 days. The effects of the composite scaffold on the cell adhesion, proliferation, morphological changes, and synthesis of the extracellular matrix were observed by scanning electronic microscopy and immunohistochemistry.
RESULTS:The chondrocytes could adhere to the surface of the scaffolds, proliferate, and migrate into the scaffolds. They maintained round and could secrete extracellular matrix on the porous scaffold.
CONCLUSION:The nHAC can be used as the cartilage cell carrier.
