Effect of heat shock protein 47 on the expression of collagen I induced by TGF-beta(1) in hepatic stellate cell-T6 cells.
- Author:
Yi LI
1
;
Wei WU
;
Yong-Fang JIANG
;
Kang-Kai WANG
Author Information
1. Institute of Hepatology, Second Xiangya Hospital, Central South University, Changsha 410011, China. chenjxly@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Cell Line;
Collagen Type I;
metabolism;
HSP47 Heat-Shock Proteins;
metabolism;
Heat-Shock Response;
Hepatic Stellate Cells;
cytology;
drug effects;
metabolism;
Humans;
Transforming Growth Factor beta1;
pharmacology
- From:
Journal of Central South University(Medical Sciences)
2007;32(4):650-655
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the effect of heat shock protein 47 (HSP47) on the expression of collagen I induced by transforming growth factor beta(1) (TGF-beta(1)) in hepatic stellate cell-T6 (HSC-T6) cells.
METHODS:We used 1 ng/mL and 10 ng/mL recombinant human TGF-beta(1) to stimulate the cultured HSC-T6 cells. Heat shock response (HSR) and antisense oligonucleotides of HSP47 were used to induce and block the expression of HSP47, respectively. The expressions of HSP47 and collagen I were detected by Western blot and the cell viability was observed by MTT assay.
RESULTS:Both HSP47 and collagen I were expressed in normal HSC-T6 cells. Collagen I and HSP47 expression could be induced by both 1 ng/mL and 10 ng/mL TGF-beta(1) and collagen I was expressed the most after the treatment with 10 ng/mL TGF-beta(1). Although HSR could not affect the synthesis of collagen I as it induced the HSP47 expression, HSR could promote the expression of collagen I induced by TGF-beta(1). With no effect on the cell viability, antisense oligonucleotides could significantly inhibit HSR-mediated HSP47 expression and TGF-beta(1)-induced collagen I synthesis.
CONCLUSION:Over-expression of HSP47 enhances TGF-beta(1)-induced expression of collagen I in HSC-T6 cells, and HSP47 may play important roles in the process of hepatic fibrosis.
