Effect of gene polymorphism of TNF-beta on the concentration of TNF in serum of patient with endometriosis.
- Author:
Min LUO
1
;
Dong-Xiang SHEN
;
Hong-Bin ZHANG
;
Jie WANG
;
Li-Li ZONG
;
Ting GUAN
;
Yuan-Li HE
Author Information
1. Department of Obstetrics and Gynecology, General Hospital of Guangzhou Military Area Command, Guangzhou 510010, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Endometriosis;
blood;
genetics;
Female;
Humans;
Lymphotoxin-alpha;
blood;
genetics;
Polymorphism, Genetic;
Tumor Necrosis Factor-alpha;
blood;
Young Adult
- From:
Journal of Central South University(Medical Sciences)
2007;32(4):656-659
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the polymorphism in +252 site of tumor necrosis factor-beta(TNF-beta) gene in patients with or without endometriosis, to evaluate the levels of TNF-alpha and TNF-beta in the serum with or without endometriosis, to explore the relation between polymorphism of TNF-beta gene and the genetic susceptibility of endometriosis, and to explore the pathogenic mechanism of endometriosis at gene level.
METHODS:By polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, polymorphism on +252 site of TNF-beta gene was measured in 82 patients with endometriosis (the endometriosis group) and 80 patients without endometriosis (the control group). With the sandwich-enzyme-linked immunosorbent assay (ELISA), the levels of TNF-alpha and TNF-beta in the serum of the two groups were determined.
RESULTS:The TNF-beta level in the serum in the endometriosis group with TNF-beta gene +252 site AA genotype significantly increased, compared with GG genotype (t=2.029, P<0.05); while TNF-alpha and TNF-beta level in the serum had no statistical significance in patients with other genotypes in TNF-beta gene +252 site in the endometriosis group and the control group.
CONCLUSION:TNF-beta gene +252 site AA genotype might be enhance TNF-beta level in the serum of patients with endometriosis.
