Effect of ulinastain on the expression of hemeoxygenase-1 in oleic acid-induced acute lung injury in rats.
- Author:
Li-Yan JIN
1
;
Jun-Mei XU
;
Zhi-Biao HE
;
Wen-Yan RUAN
;
Xiang-Ping CHAI
Author Information
1. Department of Anesthesiology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Acute Lung Injury;
chemically induced;
metabolism;
Animals;
Glycoproteins;
pharmacology;
Heme Oxygenase (Decyclizing);
metabolism;
Lung;
drug effects;
metabolism;
Male;
Oleic Acid;
adverse effects;
Rats;
Rats, Sprague-Dawley
- From:
Journal of Central South University(Medical Sciences)
2007;32(4):675-678
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of ulinastain on the expression of hemeoxy genase-1 (HO-1) in oil acid-induced acute lung injury in rats.
METHODS:The animal model of acute lung injury was established by oil acid. Thirty SD rats were randomly divided into 3 groups: the blank control group (A), the acute lung injury group (B) and the acute lung injury group (C) followed by injecting 100 mL/kg ulinastatin. Each group consisted of 10 rats. Group A were given 0.2 mL/kg natural solution through the trial vein; Group B and C were given 0.2 mL/kg oil-acid through trial vein, while group C were injected 100mL/kg ulinastatin by the peritoneal cavity after injecting oil acid. After 4 hours, the rates of respiration were counted and blood samples were cramped out through the heart puncture for blood gas analysis. The expressions of hemeoxygenase-1 and the pathologic construction changes were determined by HE staining in the lower right lung of rats in the 3 groups.
RESULTS:The respiration dysfunction caused by oil acid could be prominently improved by ulinastain. There was only a little expression of hemeoxygenase-1 in the lung of Group A, but the expression increased in Group B and significatively increased in Group C.
CONCLUSION:Ulinastatin may protect the rats from acute lung injury through increasing the expression of HO-1.
