Intrasplenic tumor model of nude mice in the anti- metastasis roles of NGX6 gene against colon cancer.
- Author:
Zhi-ming XIAO
1
;
Shou-rong SHEN
;
Ping LIAN
;
Xiao-yan WANG
;
Fen LIU
Author Information
1. Department of Gastroenterology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Colonic Neoplasms;
genetics;
pathology;
Disease Models, Animal;
Gene Expression Regulation, Neoplastic;
HT29 Cells;
Humans;
Liver Neoplasms;
pathology;
secondary;
Male;
Membrane Proteins;
genetics;
Mice;
Mice, Inbred BALB C;
Mice, Nude;
Neoplasm Metastasis;
Neoplasm Transplantation;
Splenic Neoplasms;
pathology;
Transfection;
Tumor Suppressor Proteins;
genetics
- From:
Journal of Central South University(Medical Sciences)
2007;32(5):753-757
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a liver metastasis model of nude mice in colon cancer so as to determine the function of NGX6.
METHODS:The cells of Group HT-29, pcDNA3.1(+)/HT-29, and pcDNA3.1(+)/NGX6/HT-29 were implanted into the spleen of nude mice, respectively. Everyday we measured the weight of the nude mice and observed their ingestion, movement and mental status. The nude mice were killed after 45 days, and the effect of NGX6 on the malignant behavior of HT-29 was assessed by this experiment.
RESULTS:In contrast to the other two groups, the metastasis in the liver and xenograft tumor in the spleen of pcDNA3.1(+)/NGX6/HT-29 group was significantly reduced (P<0.01).
CONCLUSION:The metastasis of HT-29 colon cancer cell line was significantly inhibited by NGX6 gene. This model of liver metastasis in the nude mice is a proper model to determine the anti-metastasis mechanism of NGX6 gene.
