Effect of calcitonin-gene-related peptide on MMP-9 production in human bronchial epithelial cells.
- Author:
Cha-xiang GUAN
1
;
Yong-ping LIU
;
Yan-ru CUI
;
Fang YU
;
Guo-ying SUN
;
Hui-jun LIU
Author Information
1. Department of Physiology, Xiangya School of Medicine, Central South University, Changsha 410078,China. guanchaxiang@xysm.net
- Publication Type:Journal Article
- MeSH:
Bronchi;
cytology;
Calcitonin Gene-Related Peptide;
pharmacology;
Calmodulin;
metabolism;
Cells, Cultured;
Epithelial Cells;
drug effects;
metabolism;
Humans;
Matrix Metalloproteinase 9;
metabolism;
Protein Kinase C;
metabolism;
Signal Transduction
- From:
Journal of Central South University(Medical Sciences)
2007;32(5):771-775
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To examine the expression of matrix metalloproteinase-9 (MMP-9) in human bronchial epithelial cells treated with calcitonin-gene-related peptide (CGRP).
METHODS:RT-PCR and gelatin zymography were performed to examine the dynamic expression and activity of MMP-9 in human bronchial epithelial cells at different doses (10(-10), 10(-9), 10(-8), 10(-7), and 10(-6)mol/L) and different time points (6,12,18,24,36, and 48h) after the stimulation of CGRP.
RESULTS:The unstimulated human bronchial epithelial cells only secreted a small amount of MMP-9. After the CGRP stimulation, the expression of MMP-9 presented in a concentration-dependent (10(-10), 10(-9), 10(-8), 10(-7), and 10(-6) mol/L) and time-dependent (6,12,18,24,36, and 48 h) manners (P<0.01) in human bronchial epithelial cells. The effect of CGRP could be diminished by H-7 and W-7, an antagonist of protein kinase C (PKC) and calmodulin (CaM) (P<0.05).
CONCLUSION:CGRP can stimulate the secretion and expression of MMP-9 in human bronchial epithelial cells, and the signal transduction is partly via the PKC and CaM pathway.
