Construction of tissue engineered gingiva with gingival epithelia and acellular dermal matrix.
- Author:
Chang-yun FANG
1
;
Xiao-li XIE
;
Bin-jie LIU
;
Zheng SU
;
Hui-li LI
;
Xiao-ming SHI
;
Ming LI
;
Na PANG
;
Wei-ying ZHONG
Author Information
1. Department of Stomatology, Xiangya Hospital, Changsha 410008, China. cyfangdental@mail.csu.edu.cn.
- Publication Type:Journal Article
- MeSH:
Acellular Dermis;
Cells, Cultured;
Connective Tissue;
Epithelial Cells;
cytology;
Gingiva;
cytology;
Humans;
Skin, Artificial;
Tissue Engineering;
methods
- From:
Journal of Central South University(Medical Sciences)
2007;32(5):796-799
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To culture human gingival epithelia in vitro, and to construct the tissue engineered gingiva with the acellular dermal matrix (ADM).
METHODS:Human gingival epithelia were isolated from the gingival tissue, and the cells were cultured and seeded onto the surface of ADM. After 7 days of submerged incubation, an air-liquid interface culture was performed for 7, 14, and 21 days. The complex constructed above was taken for histological examination.
RESULTS:Human gingival epithelia could proliferate well on the surface of ADM, and form multilayer structure. But the superficial epithelium was partially keratinized.
CONCLUSION:Tissue engineered gingiva may be constructed with human gingival epithelia and ADM in vitro.
