Effect of precursor of brain-derived neurotrophic factor on hippocampal neuron survival and its mechanism.
- Author:
Hui WANG
1
;
Dan CHEN
;
Xiao-qiong WU
;
Ju-fang HUANG
;
Xue-gang LUO
Author Information
1. Department of Human Anatomy & Neurobiology, Xiangya School of Medicine, Central South University, Changsha 410013, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Brain-Derived Neurotrophic Factor;
pharmacology;
Cell Survival;
drug effects;
Cells, Cultured;
Hippocampus;
cytology;
Mitogen-Activated Protein Kinase 1;
metabolism;
Neurons;
cytology;
metabolism;
Protein Precursors;
pharmacology;
Proto-Oncogene Proteins c-ets;
metabolism;
Proto-Oncogene Proteins c-fos;
metabolism;
Rats;
Signal Transduction
- From:
Journal of Central South University(Medical Sciences)
2007;32(5):800-805
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of precursor of brain-derived neurotrophic factor (proBDNF) on cultured hippocampal neuron and its intracellular mechanism.
METHODS:The hippocampal neurons were dissociated from E18 rats and cultured in neurobasal medium, and then the cells were treated with proBDNF, preBDNF(propeptide of proBDNF) and proBDNF antiserum,respectively. Thirty minutes, 1 hour or 48 hours later, the cells were stained with Nissl solution, and the immunocytochemistry methods of ELK-p (Ets domain protein), ErK2(extracellular signal regulated kinase) and c-fos were performed.
RESULTS:The expressions of ELK-p, ErK and c-fos were significantly upregulated in the cultured hippocampal neurons after they were treated with proBDNF protein,and the immuno-positive staining was also obvious in some nuclei. While the endogenous proBDNF was neutralized by proBDNF antiserum treatment, the expressions of ELK-p, ErK and c-fos were downregulated and many cells showed swelling and vasoculation. The immunoreactivity in preBDNF treated cells was similar to that in normal cultured cells.
CONCLUSION:proBDNF plays an important role in sustaining the hippocampal neuron survival through upregulating the ELK and ErK pathways.
