Protective effect of amlodipine on the cytotoxicity induced by contrast media in human kidney cells.
- Author:
Xiao-rong ZHOU
1
;
Shao-bin DUAN
;
You-ming PENG
;
Fu-you LIU
;
Yun YE
;
Rui-hong LIU
;
Gui-yuan LI
Author Information
1. Department of Geriatrics, Second Xiangya Hospital, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Amlodipine;
pharmacology;
Apoptosis;
drug effects;
Caspase 3;
metabolism;
Cell Line;
Contrast Media;
toxicity;
Diatrizoate Meglumine;
toxicity;
Epithelial Cells;
cytology;
drug effects;
Humans;
Kidney Tubules;
cytology;
drug effects;
Protective Agents;
pharmacology
- From:
Journal of Central South University(Medical Sciences)
2007;32(5):806-811
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the protective effect of amlodipine on the cytotoxicity induced by contrast media (meglumine diatrizoate) in human kidney cells (HKC).
METHODS:An HKC line was used. The experiment was divided into 4 groups: a model group (diatrizoate 111g/L), a prevention group (diatrizoate 111g/L+amlodipine 10(-5)mol/L), an amlodipine control group (amlodipine 10(-5)mol/L), and a culture medium control group (simple none blood serum DMEM-F12 medium). Cytotoxicity induced by meglumine diatrizoate was analysed by methyl thiazolyl tetrazolium (MTT) test, lactate dehydrogenase (LDH) assay, Hochest33258 fluorescence stained cytospins, and flow cytometric DNA analysis. The protein expression of Bax was determined by Western blot, and caspase-3 activity was examined by fluorometric method.
RESULTS:In the prevention group, the cell viability increased significantly (P<0.05), LDH levels decreased (P<0.05), and the apoptosis was lower than that of the model group (P<0.05) .Bax protein expression and caspase 3 activity decreased (P<0.05).
CONCLUSION:Amlodipine can inhibit the HKC apoptosis and protect the renal tubule cell from injury induced by meglumine diatrizoate.
