Protective effect of thrombin precondition on astrocytes insulted by oxygen-glucose deprivation.
- Author:
Xiao-ling LUO
1
;
Li-xiang WU
;
Fa-yi LIU
;
Bo-sheng HUANG
;
Fang HE
Author Information
1. Department of Physiology, Xiangya School of Medicine, Central South University, Changsha 410078.
- Publication Type:Journal Article
- MeSH:
Animals;
Animals, Newborn;
Apoptosis;
drug effects;
Astrocytes;
drug effects;
metabolism;
Cell Hypoxia;
drug effects;
Cells, Cultured;
Glucose;
metabolism;
Glutamic Acid;
metabolism;
Neuroprotective Agents;
pharmacology;
Oxygen;
metabolism;
Rats;
Rats, Sprague-Dawley;
Thrombin;
administration & dosage;
pharmacology
- From:
Journal of Central South University(Medical Sciences)
2007;32(5):845-849
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of thrombin precondition (TPC) on the rat cerebral astrocytes(As) cultured in oxygen-glucose deprivation (OGD).
METHODS:Astrocytes were pretreated with thrombin (TB) at various concentrations (0.005 approximately 5.000 kU/L), and then insulted by OGD. The cell damage and viability were evaluated by the lactate dehydrogenase (LDH) effusion rate and the 3-(4,5-dimethylthazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) conversion method. Detection of apoptotic cells was determined by the flow cytometry technique. The glutamate uptake of astrocytes was studied with [3H]-glutamate incorporation.
RESULTS:OGD increased the LDH, decreased the cell viability, increased the number of apoptotic astrocytes, and decreased the glutamate uptake (P<0.01). While preconditioned with thrombin at the same condition, the LDH decreased, the cell viability increased, the percentage of apoptotic cells decreased, and the glutamate uptake increased (P<0.05). The maximum protective effect of thrombin was observed at 0.1 kU/L.
CONCLUSION:Low concentration of thrombin precondition (TPC) can protect the astrocytes from oxygen-glucose deprived injury, and attenuate its apoptosis in a dose-dependent manner.
