Effect of ginsenoside Rh2 on immunocompetence of alveolar macrophages in patients with non-small cell lung cancer.
- Author:
Dong-bo ZHOU
1
;
Cheng-ping HU
;
Shuo LIANG
;
Hong-zhong YANG
Author Information
1. Department of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Bronchoalveolar Lavage Fluid;
chemistry;
Carcinoma, Non-Small-Cell Lung;
immunology;
Female;
Ginsenosides;
pharmacology;
Humans;
Lung Neoplasms;
immunology;
Macrophages, Alveolar;
drug effects;
immunology;
metabolism;
Male;
Middle Aged;
Nitric Oxide;
metabolism;
Tumor Cells, Cultured;
Tumor Necrosis Factor-alpha;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2007;32(5):868-872
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of ginsenoside Rh2 (G-Rh2) on the excretion of cytotoxin-effecting molecule of alveolar macrophages (AM) in patients with non-small cell lung cancer (NSCLC).
METHODS:The concentration of tumor necrosis factor (TNF-alpha) and NO in the bronchoalveolar lavage fluid (BALF) and the cultured supernatants of AM in 35 patients with NSCLC were measured by ELISA and enzyme method,and levels of TNF-alpha and NO in the cultured supernatants of AM after being cultivated with IFN-alpha, G-Rh2, and IFN-alpha+G-Rh2 were measured by the same method.
RESULTS:AM in all the non-small cell lung cancer patients produced TNF-alpha and NO. The activity of TNF-alpha and NO was lower in the BALF and in the cultured supernatants of AM of the tumor-bearing lungs than that of the non-tumor-bearing lungs. The concentrations of TNF-alpha and NO in the cultured supernatants of AM cultivated with G-Rh2 were higher than those in the control (P<0.05), but there were no significant differences between the G-Rh2 group and IFN-alpha group (P>0.05). The concentrations of TNF-alpha and NO in the cultured supernatants of AM cultivated with both G-Rh2 and IFNalpha were obviously higher than those stimulated with IFNalpha or G-Rh2 (P<0.01) alone.
CONCLUSION:G-Rh2 can enhance the excretion of cytotoxin-effecting molecules of AM in patients with NSCLC. The changes are more distinctive when G-Rh2 and IFNalpha have coordinated action.
