Bcl-2, TGFbeta1, and apoptosis in placenta tissues in patients with hypertensive disorder complicating pregnancy.
- Author:
Li-juan ZHANG
1
;
Min YANG
;
Yi-ling DING
Author Information
1. Department of Gynecology and Obstetrics, Second Xiangya Hospita, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Apoptosis;
Female;
Humans;
Hypertension, Pregnancy-Induced;
metabolism;
Placenta;
cytology;
metabolism;
Pregnancy;
Pregnancy Trimester, Third;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
Transforming Growth Factor beta1;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2007;32(5):883-889
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the apoptosis in placenta tissues of patients with hypertensive disorder complicating pregnancy and its relationship with Bcl-2, TGFbeta1, and to explore the etiology of hypertensive disorder complicating pregnancy.
METHODS:Forty-five placenta samples were obtained from pregnancies with hypertensive disorder (15 gestational hypertension, 15 mild preeclampsia, and 15 severe preeclampsia) and 45 normal placenta tissues were enrolled from the third-trimester pregnancies. Immunohistochemistry (SP method) was used to study the expression of Bcl-2 and TGFbeta1 in human trophoblasts. Terminal deoxynucleotidyl transferase-dUTP nick end-labeling (TUNEL) was used to quantify the incidence of apoptosis in human trophoblasts.
RESULTS:The apoptosis rate and TGFbeta1 expression in hypertensive disorder complicating pregnancy group was higher than that in the control group, but the Bcl-2 expression was significantly lower than the control group (all Ps<0.01). With the aggravation of this illness, the apoptosis rate and TGFbeta1 expression in the gestational hypertension group, mild preeclampsia group, and severe preeclampsia tended to be increasing, but the Bcl-2 expression was decreasing (P<0.001). The apoptosis of placenta villi and TGFbeta1 expression were positively correlated in the severe preeclampsia group and mild preeclampsia group,but the apoptosis of placenta villi and Bcl-2 were negatively correlated (all Ps<0.05). TGFbeta1 and Bcl-2 expressions in the severe preeclampsia group and mild preeclampsia group were negatively correlated (P<0.05).
CONCLUSION:Apoptosis of the placental trophoblasts of pregnancies with hypertensive disorder is evidently enhanced. The TGFbeta1 expression increases and the Bcl-2 expression decreases. The imbalance between TGFbeta1 and Bcl-2 expression may induce the hypertensive disorder.
