Apoptosis of mesenchymal cell line MBA-1 induced by core binding factor alpha 1.
- Author:
Min LIU
1
;
Hou-de ZHOU
;
Yu-lin HE
;
Hui XIE
;
Er-yuan LIAO
Author Information
1. Institute of Endocrinology and Metabolism, Second Xiangya Hospital, Central South University, Changsha 410011, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
physiology;
Bone Marrow Cells;
cytology;
Caspase 9;
Caspases;
metabolism;
Cell Line;
Core Binding Factor alpha Subunits;
pharmacology;
Cytochromes c;
metabolism;
Mesenchymal Stem Cells;
cytology;
Mice;
Protein Isoforms;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
bcl-2-Associated X Protein;
metabolism
- From:
Journal of Central South University(Medical Sciences)
2006;31(1):14-18
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of two core binding factors alpha 1 (Cbfa1) isfroms (Cbfa1/P56 and Cbfa1/P57) on the apoptosis of mesenchymal cell line MBA-1.
METHODS:The two Cbfal isfroms were transiently transfected into MBA-1 cells, then the changes of apoptosis rate were observed by flow cytometer. The protein expressions of Cbfa1, Bax, Bcl-2, caspase-3, and caspase-9, cytochrome-C and TNF-alpha were determined by Western immunoblot.
RESULTS:After the transient transfection with the two isforms of Cbfa1, MBA-1, the cells apoptotic rates increased, and the ratio of Bax/Bcl-2, the expressions of cytochrome-C, caspase-3, caspase-9, and TNF-alpha were significantly increased.
CONCLUSION:Cbfa1 can promote the apoptosis in mesenchymal cell line MBA-1. Bax/Bcl-2, cytochrome-C, caspase-9, caspase-3, and TNF-alpha are also involved in the apoptosis pathway.
