Effect of realgar on the gene expression profile of multiple myeloma cell line RPMI 8226.
- Author:
Meng-chang WANG
1
;
Shan-xi LIU
;
Peng-bo LIU
Author Information
1. Department of Hematology, First Hospital of Xi' an Jiaotong University, Xi' an, Shaanxi Province 710061, China. swallow3956@sina.com
- Publication Type:Journal Article
- MeSH:
Arsenicals;
pharmacology;
Gene Expression Profiling;
Humans;
Multiple Myeloma;
pathology;
Oligonucleotide Array Sequence Analysis;
Sulfides;
pharmacology;
Tumor Cells, Cultured
- From:
Journal of Central South University(Medical Sciences)
2006;31(1):24-27
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of realgar on the gene expression profiles of multiple myeloma cell line RPMI 8226 by apply cDNA microarray.
METHODS:The gene expression of RPMI 8226 cells before and after 48 hours of realgar treatment was determined with a cDNA microarray representing 4096 human genes.
RESULTS:At the mRNA level, 164 genes were differentially altered; 53 genes were up-regulated; and 111 genes were down-regulated.
CONCLUSION:The realgar treatment to RPMI 8226 cell line may induce a number of gene changes. Many genes may be involved in the pathogenesis of multiple myeloma. BTG1, ALK1, and TXNIP genes may play an important role in the apoptosis and differentiation of RPMI 8226 cells.
