GPI-PLD gene exon14 polymorphisms of leucocyte in peripheral blood from healthy persons and leukemia patients.
- Author:
Zhi-ying YANG
1
;
He HUANG
;
Jian-hua TANG
;
Hong YU
;
Yi-dan WANG
;
Xin-ying XIANG
Author Information
1. Department of Biochemistry, Central South University, Changsha 410078, China. miket561@xysm.net
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Base Sequence;
Exons;
genetics;
Female;
Humans;
Leukemia, Lymphocytic, Chronic, B-Cell;
genetics;
Leukemia, Myelogenous, Chronic, BCR-ABL Positive;
genetics;
Leukemia, Myeloid, Acute;
genetics;
Male;
Middle Aged;
Molecular Sequence Data;
Phospholipase D;
genetics;
metabolism;
Point Mutation;
Polymerase Chain Reaction;
Polymorphism, Genetic;
Polymorphism, Single-Stranded Conformational;
Precursor Cell Lymphoblastic Leukemia-Lymphoma;
genetics
- From:
Journal of Central South University(Medical Sciences)
2006;31(1):28-31
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To analyze the polymorphisms of glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD) gene exon 14, GPI-PLD activity of leucocyte in the peripheral blood,and the relationship in leukemia patients of Han nationality in Hunan.
METHODS:Both 96 leukemia patients and 96 healthy persons of Han nationality in Hunan were researched [including 48 acute non-lymphocytic leukaemia (ANLL) patients as group A, 31 acute lymphoblastic leukaemia (ALL) patients as group B, 12 chronic granulocytic leukaemia (CML) patients as group C, 5 chronic lymphocytic leukaemia (CLL) patients as group D]. The polymorphisms were analyzed by PCR-SSCP and sequencing;. and GPI-PLD activities were determined by GPI-anchored placental alkaline phosphatase (PLAP) as substrate and triton-X114 partioning.
RESULTS:There were four variations in the coverage of GPI-PLD gene exon 14 of leukemia patients and healthy persons. The codons of variation were: 1257 C-->T, 1298 T-->C, 1218 C-->A, 1257 C-->A. The total various frequency in leukemia patient and healthy person, which was determined by SSCP, was 28.12% and 20.83%. On the basis of the percentage of GPI-anchored PLAP conversion, the leucocyte GPI-PLD activities of the 96 leukemia patients were measured. Compared with the 96 healthy controls, the leukocyte GPI-PLD activites of ANLL and CLL patients were significantly increased; the acticities of ALL and CML patients were significantly reduced.
CONCLUSION:Leukocyte GPI-PLD gene in the peripheral blood, which belongs to healthy persons and leukemia patients of Han nationality in Hunan, is polymorphism. The leukocyte GPI-PLD activities in the four groups are remarkable.
