Protective effect of losartan on injury induced by ox-LDL in endothelial cells and the relationship with asymmetric dimethylarginine.
- Author:
Qi-ying XIE
1
;
Ze-lin SUN
;
Mei-fang CHEN
;
Tian-lun YANG
Author Information
1. Department of Cardiolog, Xiangya Hospital, Central Soutth University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Amidohydrolases;
metabolism;
Arginine;
analogs & derivatives;
analysis;
Cells, Cultured;
Endothelium, Vascular;
pathology;
Humans;
L-Lactate Dehydrogenase;
analysis;
Lipoproteins, LDL;
adverse effects;
Losartan;
pharmacology;
Nitric Oxide;
analysis;
Protective Agents;
pharmacology;
Umbilical Veins;
cytology
- From:
Journal of Central South University(Medical Sciences)
2006;31(1):66-69
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the protective effect of losartan against on injury induced by ox-LDL in endothelial cells and the relationship with asymmetric dimethylarginine (ADMA).
METHODS:Endothelial injury was induced by incubation with ox-LDL 100 mg/L in cultured HUVECs for 24 h, and the levels of ADMA, lactate dehydrogenase (LDH), nitric oxide (NO) and tumor necrosis factor-alpha (TNF-alpha) in the conditioned medium were measured. The activity of dimethylarginine dimethylaminohydrolase (DDAH) of cultured endothelial cells was also determined.
RESULTS:Incubation of endothelial cells with ox-LDL 100 mg/L for 24 h induced a marked elevation of the levels of ADMA, LDH and TNF-alpha in the conditioned medium and a significant decrease in the activity of DDAH and the content of NO (P < 0.05). Pretreatment with losartan (10(-8) - 10(-6) mmol/L) significantly inhibited the increased levels of ADMA, LDH and TNF-alpha, attenuated the decreased levels of NO and the decreased activity of DDAH induced by ox-LDL (P < 0.05).
CONCLUSION:Losartan may preserve ox-LDL-induced endothelial cell injury by increasing the DDAH activity and decreasing the ADMA level.
