Primary culture of human omental preadipocytes.
- Author:
Zhi-feng SHENG
1
;
Ping-fang WANG
;
Wei WANG
;
Jing HE
;
Wen-jing LI
Author Information
1. Institute of Metabolism and Endocrinology, Second Xiangya Hospital, Central South University, Changsha 410011, China. szf1976@hotmail.com
- Publication Type:Journal Article
- MeSH:
Adipocytes;
cytology;
Adult;
Cell Differentiation;
Cells, Cultured;
Culture Media, Conditioned;
Humans;
Male;
Omentum;
cytology
- From:
Journal of Central South University(Medical Sciences)
2006;31(1):75-78
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a primary culture method of human omental preadipocytes.
METHODS:Using enzyme-digesting method, fibroblast-like cells from the human omental adipose tissues were cultured, and then differentiated by conditional medium, and identified by oil red O staining.
RESULTS:The cultured cells were highly homogeneous, and highly proliferative in 4-5th generation. During the process of induction by conditional medium, the cells became round-like and larger, and more adipose droplets were aggregated. By oil red O staining, we confirmed the differentiated cells were mature adipocytes.
CONCLUSION:In human omental adipose tissues, there are some preadipocytes, which can differentiate into mature adipocytes with appropriate stimulus.
