Immune tolerance induced by combined heart-thymus transplantation with intrathymic inoculation of thymocytes in rats.
- Author:
Hai-bo XIONG
1
;
Sui-sheng XIA
;
Zu-fa HUANG
;
Hao WEN
;
Qi-fa YE
Author Information
1. Institute of Transplantation, Third Xiangya Hospital, Central South University, Changsha 410013, China. haiboxiong@sohu.com
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Transplantation;
Female;
Graft Rejection;
prevention & control;
Graft Survival;
Heart Transplantation;
Immune Tolerance;
Interleukin-2;
blood;
Interleukin-4;
blood;
Rats;
Rats, Sprague-Dawley;
Rats, Wistar;
Thymus Gland;
cytology;
transplantation
- From:
Journal of Central South University(Medical Sciences)
2006;31(1):85-90
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the role of allo heart and thymus transplantation by intrathymic inoculation of thymocytes.
METHODS:Wistar recipients were given intrathymic injection of allo thymocytes (2 x 10(7)) 14 days before the heart and/or thymus transplantation. Graft survival, histopathology, levels and mRNA expressions of IL-2, IL-4 in serum and cardiac-grafts were investigated.
RESULTS:Heart transplantation and heart-thymus composite transplantation with the treatment of CysA for 7 or 14 days prolonged graft survival. Heart transplantation and heart-thymus composite transplantation with intrathymic thymocytes injection induced the long-term survival of allo-grafts transiently immunosuppressed with CysA; IL-4 maintained at high levels but IL-2 kept at low levels in grafts in long-term survivals.
CONCLUSION:Intrathymic inoculation of allo thymoctyes can induce immune tolerance for both cardiac transplantation and heart-thymus combined transplantation in rats. Thymus graft may play a role in the induction and maintenance of central tolerance.
